Salmonella typhimurium interations with host cells

S. typhimurium is capable of entering into phagocytic and nonphagocytic cells and functiorting as an intracellular pathogen. During invasion of epithelial cells, S. typhimurium causes membrane rffiing and actin rearrangement as it enters. It thetx targets to a specialized vacuole where it replicates. Associated with replication is the ind.uction of a unique host filamentous structure. Isolation of S. typhimurium containing vacuoles has enabled us 1o address which host proteins are associated with the vavuolar membrane. Confocal microscopy of infected livers was used to examine the events associatetl with S. typhimurium infections in BaIb/C mice. htflamatiott, the Iocation of the bacteria, and bacterial interactions with host cells were examined in this low dose Salmonella infection model. Thi, t"chnology will aLlow us to address the role of individual virulence factors and their impact on the host during infection using a reletant model.. Suppl I 1998 Salmonella typhimurium interations with host cells A. Agneta Richter-Dahlfors, B. Brett Finlay